The Investigation of Caspase-3 and Tumor Necrosis Factor-Alpha Expression in Placentas of Patients with Preterm Premature Rupture of Membranes

Background: Caspase-3 is involved in the execution of apoptosis and widely used as an apoptotic marker. Tumor necrosis factor-α (TNF-α) released from activated macrophages has various functions such modulation cell growth differentiation, immunoregulation, coagulation, regulation endothelial function. This study investigated immunohistochemical staining caspase-3 TNF-α expression placentas pregnant women with preterm premature rupture membranes (PPROM). Methods: Placentas 25 healthy, PPROM were processed for routine histological tissue processing. stained hematoxylin-eosin, caspase-3, immunostaining. Results: Normal placental histology was observed control group. Amniotic epithelium, vascular structures, fibrinoid accumulation histologically normal. Leukocyte infiltration, thinned vessel walls dilatation congestion, syncytial nodes, increased The immune activity mainly negative components endothelium, accumulation, In group, positive reaction amniotic membrane cells, areas inflammatory infiltration. structures. nodes. Conclusions: patients showed loss weakened pathology, TNF-α. We suggest that signaling pathways can be a marker progression PPROM.